Advanced separation laboratory

Ultrahigh precision liquid chromatograph coupled with hybrid mass spectrometer

The liquid chromatograph coupled with mass spectrometer equipment LC/MS Q-TOF delivers sensitivity, resolution, mass accuracy, isotopic fidelity, and speed in one measurement. The system, couples excellent sensitivity over a wide in-spectrum dynamic range with precise quantitative accuracy, reducing the number of replicates needed or the size of a study. The LC-MS Q-TOF system is composed of the following: electrospray ionisation source (ESI), quadrupole, flight tube TOF (time of flight), collision cell and detector. Sistemul este prevăzut cu: sursă de ionizare electrospray (ESI), cuadrupol, tub de împrăștiere TOF (timp de zbor), celula de coliziune și detectorul.

Main performance characteristics of the instrument:

Flow precision better than +/- 0.07 RSD
The pump and the chromatographic system are able to sustain pressure up to 1200 bar
The pump can operate with binary gradient, with the precision of the gradient at least 0.15% RSD and the accuracy of the composition +/- 0.40%
Mass accuracy in MS mode 2 ppm, and mass accuracy in MS/MS mode 3 ppm
Mass resolution 20.000 and data acquisition speed 50 spectrums/sec;
using MassHunter software, can characterize, identify and quantify compounds in complex mixtures by MS and MS / MS
MS / MS "All Ions" technique allows undirected screening and quantification due to obtaining selective data

The liquid chromatograph coupled with mass spectrometer system is used to determine antibiotics in various matrices such as wastewater / drinking water, honey, vegetables and other foods. It is also used for the release profile of various biologically active materials.

Size exclusion chromatograph

Main performance characteristics of the instrument:

Maximum operating pressure of the chromatographic system 600 bar
Precision of the flow greater than 0,07% RSD
The chromatographic system has a degasser in order to eliminate performance losses due to gas bubbles
Injection domain 0,1-100 µL
The injector can be washed with mobile phase to reduce the possibility of cross-contamination to a minimum
The precision of injection is better than 0,25 % RSD in the 5 – 100 µL range
The multi-detector ensemble includes: refraction index detector, viscometer and light scattering detector
Column thermostat with Peltier element for cooling
Temperature range of the column is from 5 degrees under ambient temperature up to 99 degrees maximum
The chromatography software allows to determinate the absolute molecular mass, molecular mass distributions, molecular dimensions (hydrodynamic radius and gyration radius), conformation of macromolecules, analysis of copolymers, viscosity of solutions, calculation: Mn, Mw, Mz, Mz+1, Mp , Mv, universal calibration, differential and integral

Advanced separation laboratory

Ultrahigh precision liquid chromatograph coupled with hybrid mass spectrometer

Size exclusion chromatograph

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