Microbiological assessment is essential for a full range of food (including traditional food, food supplements and special foods). In this sense, the equipment specific to microbiology laboratories will be purchased, as well as established equipment that can analyze DNA, RNA or proteins, in general, to unequivocally identify the nature of the food (protein), but also the degree of denaturation. The investment in this laboratory is ~ 3.97 million RON.
Currently, accredited laboratories in Romania perform microbiological analysis of foods by classical methods based on the direct highlighting of microbial species/ strains, called microbiological indicators, whose presence and quantity indicate the risk of other biological contaminants, including pathogens agents. These methods involve the isolation and identification of microbiological parameters using special media and standardized biochemical and/or serological identification techniques. However, many biological factors (potentially pathogenic microorganisms, antibiotic-resistant microorganisms), viruses or biochemical factors (microbial toxins or different products derived from microbial metabolism) cannot be detected by classical methods.
This laboratory aims to develop and apply new methods for the detection of microbiological contaminants in food by using the latest tools and techniques. The modern methods used will complement the classical techniques and will include:
The Gallios flow cytometer is developed for the research and biopharmaceutical markets, with a strong focus on optical and electronic design, combined with powerful automation and software tools and ensures the efficient acquisition of excellent quality data up to 10 colours with advanced optical design for increased sensitivity for multicolour tests.
Capillary electrophoresis is a system developed for: protein analysis, toxicology, pharmaceutical compounds, analysis of small molecules (ions) and nucleic acids.
The system has 2 detectors included (UV-VIS and DAD) and can be upgraded with other detectors such as LIF.
This system can be coupled with the mass spectrometer, the data being collected and processed using the included software.
Characteristics:
Absorbance
Optical range: 230-1000 nm;
Wavelength bandwidth: 4 nm;
Wavelength accuracy: ± 2 nm;
Wavelength repeatability: ± 1 nm;
Photometric range: 0-4 OD;
Photometric resolution: 0.001 OD;
Photometric resolution: 0.001 OD;
Photometric precision: <± 0.010 OD ± 1%, 0-2 OD;
Stray light: <0.05% at 230 nm.
Fluorescence
Wavelength range: 250-850 nm;
Wavelength selection: 1nm;
Adjustable bandwidth: EX-9/15 nm and EM-15/25 nm;
Linear dynamic range:> 6 orders of magnitude;
Sensitivity: 0.5 pM in 96 plate and 1 pM in 384 plate;
Luminescence:
Wavelength range: 300-850 nm;
Wavelength selection: 1 nm and the possibility of detecting several wavelengths simultaneously;
Linear dynamic range:> 6 orders of magnitude;
Sensitivity: 15 pM in 96 plate and 30 pM in 384 plate;
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